Selection and Validation of Reference Genes for Gene Expression Studies by RT-PCR in Dalbergia odori
时间:2020-06-11 17:31:29
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Selection and Validation of Reference Genes for Gene Expression Studies by RT-PCR in Dalbergia odorifera
作 者:HuiMeng, YunYang, Zhi-HuiGao* & Jian-HeWei*
影响因子: 4.011
刊物名称: Scientific Reports
出版年份: 2019
卷:    期: 9   页码: 3341-3341

文章摘要 :

Perennial tree Dalbergia odorifera T. Chen could form the precious heartwood used to produce chinese
traditional medicine, rosewood furniture and fragrances. However the formation of heartwood is time
consuming and low efcient, leading to the severe destruction of its wild resources. Thus, it is urgent
to study the molecular mechanism of heartwood formation in D. odorifera. But till now, there is no
report about the reference gene selection in this species. In this study, the expression stability of nine
candidate reference genes were evaluated across diferent tissues and stems treated by wound and
chemical stimulators. Four algorithms were applied to obtain the robust genes. The results support
HIS2, GAPDH, and CYP to be the most stable reference genes in samples under diferent wound
treatments while DNAj was the least stable. In diferent tissues, HIS2, UBQ, and RPL were the most
stable reference genes while DNAj was the least stable. The selected reference genes were validated
through the normalization of the qRT-PCR data of six heartwood related genes in terpene biosynthesis
pathway and ethylene signal pathway. The results showed that their expression levels were accurate
when they were normalized by the most stable reference gene HIS2, or by the combination of the two
or three most stable reference genes. These results demonstrated that these selected reference genes
are reliable.